- Record: found
- Abstract: not found
- Article: not found
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
March 11 1983
March 11 1983
There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
We have developed a procedure for preparing extracts from nuclei of human tissue culture
cells that directs accurate transcription initiation in vitro from class II promoters.
Conditions of extraction and assay have been optimized for maximum activity using
the major late promoter of adenovirus 2. The extract also directs accurate transcription
initiation from other adenovirus promoters and cellular promoters. The extract also
directs accurate transcription initiation from class III promoters (tRNA and Ad 2
VA).