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Retrotransposon-based sequence-specific amplification polymorphism markers reveal that cultivated Pyrus ussuriensis originated from an interspecific hybridization

, , , , , Department of Horticulture, The State Agricultural Ministry Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou 310058, Zhejiang, China, Forest & Fruit Tree Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China , Horticultural Sub-Academy, Heilongjiang Academy of Agricultural Sciences, Harbin 150069, Heilongjiang, China, Department of Horticulture, The State Agricultural Ministry Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou 310058, Zhejiang, China, Department of Horticulture, The State Agricultural Ministry Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou 310058, Zhejiang, China

European Journal of Horticultural Science

International Society for Horticultural Science (ISHS)

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      Phylogeny and classification of Rosaceae

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        Genetic distribution of Bare-1-like retrotransposable elements in the barley genome revealed by sequence-specific amplification polymorphisms (S-SAP).

        Retrotransposons are present in high copy number in many plant genomes. They show a considerable degree of sequence heterogeneity and insertional polymorphism, both within and between species. We describe here a polymerase chain reaction (PCR)-based method which exploits this polymorphism for the generation of molecular markers in barley. The method produces amplified fragments containing a Bare-1-like retrotransposon long terminal repeat (LTR) sequence at one end and a flanking host restriction site at the other. The level of polymorphism is higher than that revealed by amplified fragment length polymorphism (AFLP) in barley. Segregation data for 55 fragments, which were polymorphic in a doubled haploid barley population, were analysed alongside an existing framework of some 400 other markers. The markers showed a widespread distribution over the seven linkage groups, which is consistent with the distribution of the Bare-1 class of retrotransposons in the barley genome based on in situ hybridisation data. The potential applicability of this method to the mapping of other multicopy sequences in plants is discussed.
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          Genetic diversity and similarity of pear (Pyrus L.) cultivars native to East Asia revealed by SSR (simple sequence repeat) markers

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            Author and article information

            Journal
            European Journal of Horticultural Science
            Europ.J.Hortic.Sci.
            International Society for Horticultural Science (ISHS)
            16114426
            16114434
            October 30 2016
            October 26 2016
            October 30 2016
            October 26 2016
            : 81
            : 5
            : 264-272
            10.17660/eJHS.2016/81.5.5
            © 2016
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