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      A DNA microarray for identification of selected Korean birds based on mitochondrial cytochrome c oxidase I gene sequences.

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          Abstract

          DNA barcoding with the gene encoding cytochrome c oxidase I (COI) in the mitochondrial genome has been proposed as a standard marker to identify and discover animal species. Some migratory wild birds are suspected of transmitting avian influenza and pose a threat to aircraft safety because of bird strikes. We have previously reported the COI gene sequences of 92 Korean bird species. In the present study, we developed a DNA microarray to identify 17 selected bird species on the basis of nucleotide diversity. We designed and synthesized 19 specific oligonucleotide probes; these probes were arrayed on a silylated glass slide. The length of the probes was 19-24 bps. The COI sequences amplified from the tissues of the selected birds were labeled with a fluorescent probe for microarray hybridization, and unique hybridization patterns were detected for each selected species. These patterns may be considered diagnostic patterns for species identification. This microarray system will provide a sensitive and a high-throughput method for identification of Korean birds.

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          Author and article information

          Journal
          Mol. Cells
          Molecules and cells
          Springer Nature America, Inc
          0219-1032
          1016-8478
          Oct 2010
          : 30
          : 4
          Affiliations
          [1 ] Department of Biochemistry, Hanyang University and GenoCheck Co. Ltd., Ansan, 426-791, Korea.
          Article
          10.1007/s10059-010-0118-8
          20821060
          5d35f9e5-6447-4a64-8897-cc394b7a9f24
          History

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