A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers.

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Abstract

In this paper, carbon nanotubes (CNTs) were successfully incorporated in the composite composed of hemoglobin (Hb) and collagen using co-electrospinning technology. The formed Hb-collagen-CNTs composite nanofibers possessed distinct advantage of three-dimensional porous structure, biocompatibility and excellent stability. The Hb immobilized in the electrospun nanofibers retained its natural structure and the heterogeneous electron transfer rate constant (ks) of the direct electron transfer between Hb and electrodes was 5.3s(-1). In addition, the electrospun Hb-collagen-CNTs nanofibers modified electrodes showed good electrocatalytic properties toward H2O2 with a detection limit of 0.91μM (signal-to-noise ratio of 3) and the apparent Michaelis-Menten constant (Km(app)) of 32.6μM.

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Journal

Journal ID (iso-abbrev): Colloids Surf B Biointerfaces

Title: Colloids and surfaces. B, Biointerfaces

Publisher: Elsevier BV

ISSN (Electronic): 1873-4367

ISSN (Print): 0927-7765

Publication date (Electronic): Jun 01 2014

Volume: 118

Affiliations

[1 ] Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin, 150080, China. Electronic address: lijia@hrbust.edu.cn.

[2 ] Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin, 150080, China.

[3 ] Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China. Electronic address: zhengwei75@126.com.

[4 ] Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China.

[5 ] Center for Biomedical Materials and Engineering, Harbin Engineering University, Harbin 150001, China; Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China.

Article

Publisher Item ID: S0927-7765(14)00167-2

DOI: 10.1016/j.colsurfb.2014.03.035

PubMed ID: 24732396

SO-VID: 5d5f7280-3b19-491d-bbad-1b9c57a14aa4

History

Keywords: Electrochemistry,Hemoglobin-collagen-CNTs nanofibers,Biosensor,Electrospinning

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Keywords: Electrochemistry, Hemoglobin-collagen-CNTs nanofibers, Biosensor, Electrospinning

A novel hydrogen peroxide biosensor based on hemoglobin-collagen-CNTs composite nanofibers.

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Abstract

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Electrospinning for biomedical applications

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