Effect of cryptorchidism in the ram: changes in the concentrations of testosterone and estradiol and receptors for LH and FSH in the testis, and its histology

Abstract

Cryptorchidism was induced in 5 pre-pubertal lambs and 7 adult rams, 5 months after surgery, testicular weight and membrane protein content were 4-fold lower than in the control. The total number of Leydig cells per testis was markedly decreased but their size was not changed. In contrast, the total number of Sertoli cells per testis was not affected but their nuclear size was smaller. Induced cryptorchidism had no effect on the length of seminiferous tubules; blood vessel volume was reduced; and the production of germ cells was completely disrupted. The number of LH receptors estimated per Leydig cell was not changed in pre-pubertal lambs but decreased 4-fold in adult rams. The number of FSH receptors calculated per Sertoli cell was reduced by 95% in both pre-pubertal and adult animals. No effect on the binding affinities of LH (Ka = 1 X 10(10) M-1) and FSH (Ka = 4.5 X 10(9) M-1) to their testicular receptors was observed. Although testicular concentrations of testosterone and estradiol-17 beta were increased, the total content of testosterone within the testis was increased only in pre-pubertal lambs. The estimated ratio of testosterone per Leydig cell was higher in cryptorchid animals than in controls, suggesting that, despite their reduction in number and the decrease of LH receptors, the Leydig cells of cryptorchid rams have an enhanced steroidogenic capacity. This study also confirms the important dysfunction of the Sertoli cells in cryptorchid rams.