30
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Extraction and Quantitative HPLC Analysis of Coumarin in Hydroalcoholic Extracts of Mikania glomerata Spreng: ("guaco") Leaves

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Methods for preparation of hydroalcoholic extracts of "guaco" (Mikania glomerata Spreng.) leaves were compared: maceration, maceration under sonication, infusion and supercritical fluid extraction. Evaluation of these methods showed that maceration under sonication had the best results, when considering the ratio extraction yield/extraction time. A high performance liquid chromatography (HPLC) procedure for the determination of coumarin in these hydroalcoholic extracts of "guaco" leaves is described. The HPLC method is shown to be sensitive and reproducible.

          Translated abstract

          Foram avaliados vários métodos de extração para os extratos hidroalcoólicos das folhas de "guaco" (Mikania glomerata Spreng.): maceração, maceração com ultra-som, infusão e extração por fluido supercrítico. Dentre os métodos estudados, a maceração com ultra-som proporcionou os melhores resultados, especialmente considerando-se a relação rendimento da extração/ tempo de extração. A cromatografia líquida de alta eficiência (CLAE) foi utilizada para determinar a concentração de cumarina nos extratos hidroalcoólicos das folhas de "guaco". A metodologia de análise desenvolvida utilizando CLAE mostrou ser sensível e reprodutível.

          Related collections

          Most cited references8

          • Record: found
          • Abstract: not found
          • Book: not found

          Pharmacopeia dos Estados Unidos do Brasil

            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Gas and liquid chromatography in analytical chemistry

              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Anal. Chem.

                Bookmark

                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Journal
                jbchs
                Journal of the Brazilian Chemical Society
                J. Braz. Chem. Soc.
                Sociedade Brasileira de Química (São Paulo )
                1678-4790
                December 2001
                : 12
                : 6
                : 706-709
                Affiliations
                [1 ] Universidade de São Paulo Brazil
                Article
                S0103-50532001000600003
                10.1590/S0103-50532001000600003
                5dd4d640-adb8-43d1-854e-f102e317dfe1

                http://creativecommons.org/licenses/by/4.0/

                History
                Product

                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0103-5053&lng=en
                Categories
                CHEMISTRY, MULTIDISCIPLINARY

                General chemistry
                Mikania glomerata Spreng (Compositae),coumarin,high performance liquid chromatography (HPLC),extraction techniques

                Comments

                Comment on this article