21
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Light Stimulates a Transducin-Independent Increase of Cytoplasmic Ca 2+ and Suppression of Current in Cones from the Zebrafish Mutant nof

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Transducins couple visual pigments to cGMP hydrolysis, the only recognized phototransduction pathway in vertebrate photoreceptors. Here we describe a zebrafish mutant, no optokinetic response f w21 ( nof), with a nonsense mutation in the gene encoding the α subunit of cone transducin. Retinal morphology and levels of phototransduction enzymes are normal in nof retinas, but cone transducin is undetectable. Dark current in nof cones is also normal, but it is insensitive to moderate intensity light. The nof cones do respond, however, to bright light. These responses are produced by a light-stimulated, but transducin-independent, release of Ca 2+ into the cone cytoplasm. Thus, in addition to stimulating transducin, light also independently induces release of Ca 2+ into the photoreceptor cytoplasm.

          Related collections

          Author and article information

          Journal
          J Neurosci
          J. Neurosci
          jneuro
          jneurosci
          J. Neurosci
          The Journal of Neuroscience
          Society for Neuroscience
          0270-6474
          1529-2401
          15 January 2003
          : 23
          : 2
          : 470-480
          Affiliations
          [ 1 ]Departments of Biochemistry and
          [ 2 ]Physiology and Biophysics, University of Washington, Seattle, Washington 98195,
          [ 3 ]Physiological Laboratory, University of Cambridge, Cambridge CB2 3EG, United Kingdom, and
          [ 4 ]Departments of Physiological Science and Ophthalmology, University of California Los Angeles, Los Angeles, California 90095-1606
          Article
          PMC6741873 PMC6741873 6741873 7193
          10.1523/JNEUROSCI.23-02-00470.2003
          6741873
          12533607
          5df5df1b-0a90-4f28-9021-1e619b05a157
          Copyright © 2003 Society for Neuroscience
          History
          : 2 August 2002
          : 21 October 2002
          : 24 October 2002
          Categories
          ARTICLE
          Cellular/Molecular
          Custom metadata
          5.00

          light adaptation , photoreceptor mutations , G-protein-mediated signal transduction , genetic analysis of phototransduction , transducin , cone photoreceptor physiology

          Comments

          Comment on this article