0
views
0
recommends
+1 Recommend
1 collections
    0
    shares

      Why publish your research Open Access with G3: Genes|Genomes|Genetics?

      Learn more and submit today!

      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Identification of the sex chromosome system in a sand fly species, Lutzomyia longipalpis s.l

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          In many animal species, sex determination is accomplished by heterogamety i.e., one of the sexes produces two types of gametes, which upon fertilization will direct the development toward males or females. Both male (“XY”) and female (“ZW”) heterogamety are known to occur and can be easily distinguished when the sex-chromosomes are morphologically different. However, this approach fails in cases of homomorphic sex chromosomes, such as the sand fly Lutzomyia longipalpis s.l. (Psychodidae, Diptera), which is the main vector of visceral leishmaniosis in Brazil. In order to identify the heterogametic sex in L. longipalpis s.l., we did a whole-genome sequencing of males and females separately and used the “Y chromosome Genome Scan” (YGS) method to find sex-specific sequences. Our results, which were confirmed by PCR, show that L. longipalpis s.l. has XY system. The YGS method can be especially useful in situations in which no morphological difference is observed in the sex-chromosomes or when fresh specimens are not readily available.

          Related collections

          Most cited references49

          • Record: found
          • Abstract: found
          • Article: not found

          SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing.

          The lion's share of bacteria in various environments cannot be cloned in the laboratory and thus cannot be sequenced using existing technologies. A major goal of single-cell genomics is to complement gene-centric metagenomic data with whole-genome assemblies of uncultivated organisms. Assembly of single-cell data is challenging because of highly non-uniform read coverage as well as elevated levels of sequencing errors and chimeric reads. We describe SPAdes, a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler (specialized for single-cell data) and on popular assemblers Velvet and SoapDeNovo (for multicell data). SPAdes generates single-cell assemblies, providing information about genomes of uncultivatable bacteria that vastly exceeds what may be obtained via traditional metagenomics studies. SPAdes is available online ( http://bioinf.spbau.ru/spades ). It is distributed as open source software.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs.

            Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              GenomeScope: fast reference-free genome profiling from short reads.

              GenomeScope is an open-source web tool to rapidly estimate the overall characteristics of a genome, including genome size, heterozygosity rate and repeat content from unprocessed short reads. These features are essential for studying genome evolution, and help to choose parameters for downstream analysis. We demonstrate its accuracy on 324 simulated and 16 real datasets with a wide range in genome sizes, heterozygosity levels and error rates.
                Bookmark

                Author and article information

                Contributors
                Role: Editor
                Journal
                G3 (Bethesda)
                Genetics
                g3journal
                G3: Genes|Genomes|Genetics
                Oxford University Press
                2160-1836
                August 2021
                26 June 2021
                26 June 2021
                : 11
                : 8
                : jkab217
                Affiliations
                [1 ] Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, CCS , Rio de Janeiro sl A2-075 21941-971, Brazil
                [2 ] Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, FIOCRUZ , Rio de Janeiro, Brazil
                Author notes
                Corresponding author: Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-971, Brazil. Email: fvigoder@ 123456gmail.com
                Author information
                https://orcid.org/0000-0003-1037-8851
                https://orcid.org/0000-0001-8959-6469
                Article
                jkab217
                10.1093/g3journal/jkab217
                8496290
                5e72eebb-1c3d-4e0d-9f83-dddf576f1006
                © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 04 February 2021
                : 10 June 2021
                Page count
                Pages: 7
                Funding
                Funded by: Welcome Trust;
                Award ID: 207486/Z/17/Z
                Categories
                Investigation
                AcademicSubjects/SCI01180
                AcademicSubjects/SCI01140
                AcademicSubjects/SCI00010
                AcademicSubjects/SCI00960

                Genetics
                sand flies,sex chromosome system,y chromosome,ygs,lutzomyia longipalpis,psychodidae: diptera,genetics of sex

                Comments

                Comment on this article