6
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Identifying the cellular targets of natural products using T7 phage display.

      1 , 1
      Natural product reports
      Royal Society of Chemistry (RSC)

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Covering: up to the end of 2015While Nature continues to deliver a myriad of potent and structurally diverse biologically active small molecules, the cellular targets and modes of action of these natural products are rarely identified, significantly hindering their development as new chemotherapeutic agents. This article provides an introductory tutorial on the use of T7 phage display as a tool to rapidly identify the cellular targets of natural products and is aimed specifically at natural products chemists who may have only limited experience in molecular biology. A brief overview of T7 phage display is provided, including its strengths, weaknesses, and the type of problems that can and cannot be tackled with this technology. Affinity probe construction is reviewed, including linker design and natural product derivatisation strategies. A detailed description of the T7 phage biopanning procedure is provided, with valuable tips for optimising each step in the process, as well as advice for identifying and avoiding the most commonly encountered challenges and pitfalls along the way. Finally, a brief discussion is provided on techniques for validating the cellular targets identified using T7 phage display.

          Related collections

          Author and article information

          Journal
          Nat Prod Rep
          Natural product reports
          Royal Society of Chemistry (RSC)
          1460-4752
          0265-0568
          May 04 2016
          : 33
          : 5
          Affiliations
          [1 ] Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia. peter.karuso@mq.edu.au.
          Article
          10.1039/c5np00128e
          26964751
          5ef885bb-bf0f-46cb-930f-bfd4e337e92f
          History

          Comments

          Comment on this article