A stress associated NAC transcription factor MpSNAC67 from banana (Musa x paradisiaca) is involved in regulation of chlorophyll catabolic pathway

Abstract

Process of senescence includes multiple steps involving break-down of chlorophyll to degrade photosynthetic machinery. In this study, we showed that a stress-associated NAC transcription factor MpSNAC67 regulates senescence by promoting chlorophyll-catabolic genes. MpSNAC67 encodes a transcriptional activator and its promoter activity is restricted to vascular tissue of banana. Expression of MpSNAC67 showed positive responses to multiple abiotic stress conditions suggesting that MpSNAC67 is a stress associated NAC transcription factor. Transgenic banana lines overexpressing MpSNAC67 showed highly senesced phenotype including yellowing and de-greening of leaves similar to etiolated leaves. Transgenic leaves possessed low chlorophyll content and failed to retain normal chloroplast morphology including loss of granum thylakoid, non-uniform chloroplast membrane and increased number as well as size of plastoglobulins. In a gel shift assay MpSNAC67 could retard the mobility of chlorophyll catabolic genes such as PAO-like (Pheophorbide-a-oxygenase), HCAR-like (hydroxymethyl chlorophyll-a-reductase), NYC/NOL-like (Chlorophyll-b-reductase) as well as ORS1-like (a SenNAC). Expression of these genes were highly elevated in transgenic lines which indicate that MpSNAC67 is a positive regulator of senescence in banana and exercise its effect by regulating the expression of chlorophyll catabolic genes and ORS1.