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      In vitro and in vivo effects of xanthorrhizol on human breast cancer MCF-7 cells treated with tamoxifen.

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          Abstract

          This study investigated the herb-drug interaction of xanthorrhizol and tamoxifen in human breast cancer cells. Using MCF-7 cell line as an in vitro model, the herb-drug interaction between xanthorrhizol and tamoxifen was measured by MTT assay, luciferase reporter assay, and cell cycle analysis. The effects of xanthorrhizol on growth/autophagy related signaling were determined by immunostaining, western blotting, and real time RT-PCR. Additionally, the in vivo effect of xanthorrhizol and tamoxifen on athymic nude mice implanted with MCF-7 cells was evaluated. When MCF-7 cells were co-treated with tamoxifen and xanthorrhizol, there were no significant changes in terms of cell number, luciferase activity, percentage S-phase cells and LC3-II expression. However, using the MCF-7 implanted nude mice model, it was possible to detect significantly increased tumor volumes, a larger tumor size, and increased protein expression of P38 and P27(Kip1) in the xanthorrhizol + tamoxifen group compared to the tamoxifen-alone group. It can be concluded that while there is no significant herb-drug interaction between xanthorrhizol and tamoxifen in vitro, there is such an interaction in tumor-bearing mice, which provides important information that affects breast cancer treatment translational research.

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          Author and article information

          Journal
          J. Pharmacol. Sci.
          Journal of pharmacological sciences
          1347-8648
          1347-8613
          2014
          : 125
          : 4
          Affiliations
          [1 ] Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taiwan.
          Article
          DN/JST.JSTAGE/jphs/14024FP
          10.1254/jphs.14024FP
          25141924
          5f4144b0-30b0-4d30-8f2f-61a90f0ebd58
          History

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