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      Poly (2-methyl-2-oxazoline) coating by thermally induced immobilization for determination of bovine lactoferrin in infant formula with capillary electrophoresis.

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          Abstract

          In this work, a one-step coating procedure by a simple annealing protocol of poly (2-methyl-2-oxazoline)-random-glycidyl methacrylate (PMOXA-r-GMA) copolymer was used to yield covalent and cross-linked PMOXA-based antifouling coating on a fused-silica capillary inner surface, which was used to determine the bovine lactoferrin (Lf) in infant formula by capillary electrophoresis with ultraviolet (CE-UV) detection. The X-ray photoelectron spectroscopy (XPS) confirmed that PMOXA-r-GMA could be bonded onto fused-silica capillary inner wall and stable electroosmotic flow (EOF) was obtained in the PMOXA-r-GMA coated capillary at pH 2.2-9.0. The separation of a mixture of four basic proteins indicated that the PMOXA-r-GMA coated capillary exhibited excellent separation efficiency for the basic proteins. Therefore, the PMOXA-r-GMA coated capillary was used to determine the quantity of Lf in infant formula. Under the optimal conditions, the peak area (A) and the concentration of Lf showed a good linear relationship within the range of 10-500μg/mL with a linear correlation coefficient of 0.9995. The limit of detection (LOD) and limit of quantitation (LOQ) for Lf were 5.0μg/mL and 16.7μg/mL, respectively. The recoveries at spiked concentrations of 0.20 and 0.40mg/mL Lf in infant formula were 97.1±5.5% - 97.8±5.1%. The determined values of Lf in infant formula samples with Lf were consistent with the nominal values, indicating that our CE method could be successfully applied to the quantitative analysis of Lf in commercial infant formula.

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          Author and article information

          Journal
          Talanta
          Talanta
          Elsevier BV
          1873-3573
          0039-9140
          Jun 01 2017
          : 168
          Affiliations
          [1 ] CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, PR China.
          [2 ] CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, PR China. Electronic address: wangyanm@ustc.edu.cn.
          Article
          S0039-9140(17)30339-9
          10.1016/j.talanta.2017.03.051
          28391848
          5f5bf53f-d70b-4f24-b3b1-921594a35ba8
          History

          Capillary electrophoresis,Infant formula,Lactoferrin,Poly (2-methyl-2-oxazoline)-random-glycidyl methacrylate,Thermally induced immobilization

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