A Radioimmunoassay Method for Measurement of Urinary Kinins

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

A sensitive and specific radioimmunoassay for measuring urinary kinins was developed. Antibodies against bradykinin were induced in rabbits by injecting bradykinin coupled to bovine albumin. One of the antisera generated was used at a final dilution of 1:18,000 to obtain a 30% total binding of bradykinin-(8-tyrosine)-[<sup>125</sup>I]-triacetate. Synthetic bradykinin (5–1,000 pg) was used as standard in the curves. The sensitivity of the assay was 5 pg. The recovery of bradykinin added to urinary samples was 86.85 ± 6%. The intraassay and interassay coefficients of variation were 3.3% (n = 12) and 4.4% (n = 5), respectively. The antiserum showed no cross-reactivity with oxytocin or low molecular weight kininogen and cross-reacted with kallidin (lys-bradykinin), met-kallidin, and angiotensin I, but cross-reaction with angiotensin I (2.5%) was low enough to be disregarded. The mean urinary levels of total kinins in 12 normal subjects were 23.2 ± (SEM) 2.2 µg/day.

Related collections

Author and article information

Journal

Journal ID (publisher-id): HRE

Journal ID (doi): 10.1159/issn.0018-5051

Title: Hormone Research in Paediatrics

Publisher: S. Karger AG

ISSN (Print): 0018-5051

ISSN (Electronic): 2571-6603

Publication date Subscription-year: 1984

Publication date (Print): 1984

Publication date (Electronic): 26 November 2008

Volume: 20

Issue: 2

Pages: 143-149

Affiliations

Cátedra de Endocrinología Experimental, Departamento de Fisiología, Facultad de Medicina, Universidad Complutense, Madrid, España

Article

Publisher ID: 179987 Other ID: Horm Res 1984;20:143–149

DOI: 10.1159/000179987

SO-VID: 5f8e85a2-0da3-4b23-bc0b-f36637ce3b15

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 05 May 1983

Date accepted : 30 January 1984

Page count

Pages: 7

Comments

Comment on this article

scite_