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      Correction: Reproducibility of Fluorescent Expression from Engineered Biological Constructs in E. coli

      correction
      , , , , , , , iGEM Interlab Study Contributors
      PLoS ONE
      Public Library of Science

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          Abstract

          The Acknowledgments contain several errors in the “2014 iGEM Interlab Study Contributors” section. In the “ETH_Zurich” subsection, the correct spelling of “Verena Jagger” is “Verena Jäggin”. In the “HUST-China” subsection, the correct spelling of “Yunjun Yang” is “Yunjun Yan”. In the “Leicester” subsection, the name “Ross Campbell” should be listed after “Richard Badge”. In the “Marburg” subsection, the name “Daniel F. Hurtgen” should be listed after “Matthias Franz”.

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          Reproducibility of Fluorescent Expression from Engineered Biological Constructs in E. coli

          We present results of the first large-scale interlaboratory study carried out in synthetic biology, as part of the 2014 and 2015 International Genetically Engineered Machine (iGEM) competitions. Participants at 88 institutions around the world measured fluorescence from three engineered constitutive constructs in E. coli. Few participants were able to measure absolute fluorescence, so data was analyzed in terms of ratios. Precision was strongly related to fluorescent strength, ranging from 1.54-fold standard deviation for the ratio between strong promoters to 5.75-fold for the ratio between the strongest and weakest promoter, and while host strain did not affect expression ratios, choice of instrument did. This result shows that high quantitative precision and reproducibility of results is possible, while at the same time indicating areas needing improved laboratory practices.
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            Author and article information

            Journal
            PLoS One
            PLoS ONE
            plos
            plosone
            PLoS ONE
            Public Library of Science (San Francisco, CA USA )
            1932-6203
            3 June 2016
            2016
            : 11
            : 6
            : e0157255
            Article
            PONE-D-16-21468
            10.1371/journal.pone.0157255
            4892490
            27258546
            6081b46c-d66f-4441-9f0a-5848a29b2ee7
            © 2016 Beal et al

            This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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