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      Structure of the sulfakinin cDNA and gene expression from the Mediterranean field cricket Gryllus bimaculatus.

      Insect Molecular Biology
      Amino Acid Sequence, Animals, Base Sequence, Bees, genetics, DNA, Complementary, Diptera, Gene Expression Regulation, Gryllidae, metabolism, Molecular Sequence Data, Neuropeptides

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          Abstract

          The sulfakinins are multifunctional insect neuropeptides displaying sequence similarities with the gastrin/ cholecystokinin (CCK) peptide family. In vertebrates, the peptides gastrin and CCK are involved in the regulation of digestion and food-intake. In this study sulfakinin cDNA was cloned and sequenced from the Mediterranean field cricket Gryllus bimaculatus. The cDNA encodes two peptides flanked by endoproteolytic processing sites, designated GrybiSKI (QSDDYGHMRFG) and GrybiSKII (EPFDDYGHMRFG). The peptides include the characteristic amino acid Tyr, which is potentially sulphated, and a Gly, as a recognition site for amidation yeilding the common C-terminal amino acid sequence of the sulfakinin peptide family. RT-PCR studies indicate an expression of the gene restricted to the brain, with a constant level of expression throughout the last larval stage, but showing an age-dependent decrease of expression in adult females.

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          Author and article information

          Journal
          17488300
          10.1111/j.1365-2583.2007.00737.x

          Chemistry
          Amino Acid Sequence,Animals,Base Sequence,Bees,genetics,DNA, Complementary,Diptera,Gene Expression Regulation,Gryllidae,metabolism,Molecular Sequence Data,Neuropeptides

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