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      Light and transmission electron microscopy of Cepedea longa (Opalinidae) from Fejervarya limnocharis Translated title: Microscopie photonique et électronique à transmission de Cepedea longa (Opalinidae) de Fejervarya limnocharis

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      1 , 1 , 2 , * , 2 , 2 , 2 , 2
      Parasite
      EDP Sciences
      Cepedea longa, Fejervarya limnocharis, Morphology, Opalinid, Ultrastructure

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          Abstract

          Cepedea longa Bezzenberger, 1904, collected from Fejervarya limnocharis (Amphibia, Anura, Ranidae) from Honghu Lake, Hubei Province, China in May–July 2016, is described at both light and transmission electron microscope levels. This is the first electron microscopic study of this species. Cepedea longa possesses a developed fibrillar skeletal system, composed of longitudinal fibrillar bands and transversal fibrils as well as numerous thin microfibrils dispersed in the endoplasm, which may play an important role in morphogenesis and offer some resilience to deformations of the cell. Longitudinal microfibrils are polarizing elements of kineties, bordering the somatic kineties on the left side and possibly responsible for kinetosome alignment. Two types of vesicles exist in the somatic cortex: globular endocytotic vesicles and flattened exocytotic vesicles. As to the nuclei of C. longa, a thick microfibrillar layer was observed to attach to the cytoplasmic face of the nuclear envelope. This fact suggests no necessary connection between the presence of this microfibrillar layer and the number of nuclei. In addition, some unknown tightly-packed microtubular structures in the nucleoplasm were observed for the first time in opalinids; neither their nature nor physiological significance is known. A detailed list of all reported Cepedea species is included.

          Translated abstract

          Cepedea longa Bezzenberger, 1904, prélevé chez Fejervarya limnocharis (Amphibia, Anura, Ranidae) du lac Honghu, province du Hubei en mai-juillet 2016, est décrit au microscope photonique et au microscope électronique à transmission. Il s’agit de la première étude au microscope électronique de cette espèce. Cepedea longa possède un système squelettique fibrillaire développé, composé de bandes fibrillaires longitudinales et de fibrilles transversales ainsi que de nombreuses microfibrilles minces dispersées dans l’endoplasme, qui peuvent jouer un rôle important dans la morphogenèse et offrir une certaine résilience aux déformations de la cellule. Les microfibrilles longitudinales sont des éléments polarisants des kinéties, bordant les kinéties somatiques du côté gauche et éventuellement responsables de l’alignement kinétosomique. Deux types de vésicules existent dans le cortex somatique : des vésicules endocytotiques globulaires et des vésicules exocytotiques aplaties. Quant aux noyaux de C. longa, une couche microfibrillaire épaisse a été observée, qui se fixe à la face cytoplasmique de l’enveloppe nucléaire. Ce fait ne suggère aucun lien nécessaire entre la présence de cette couche microfibrillaire et le nombre de noyaux. De plus, on a observé pour la première fois chez les opalinidés des structures microtubulaires étroitement entassées dans le nucléoplasme, mais on ne connaît ni leur nature ni leur signification physiologique. Une liste détaillée de toutes les espèces de Cepedea est incluse.

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          Most cited references55

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          Human parasite finds taxonomic home.

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            The Opalinid Infusorians: Flagellates or Ciliates?

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              Further studies on the opalinid ciliate infusorians and their hosts

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                Author and article information

                Journal
                Parasite
                Parasite
                parasite
                Parasite
                EDP Sciences
                1252-607X
                1776-1042
                2017
                01 February 2017
                : 24
                : ( publisher-idID: parasite/2017/01 )
                : 6
                Affiliations
                [1 ] Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University Wuhan 430023 PR China
                [2 ] Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences Wuhan 430072 PR China
                Author notes
                [* ]Corresponding author: liming@ 123456ihb.ac.cn
                Article
                parasite160090 10.1051/parasite/2017006
                10.1051/parasite/2017006
                5780805
                28145223
                60d1a18f-b9d5-4da8-bac1-043ddcdb5dd6
                © C. Li et al., published by EDP Sciences, 2017

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 21 October 2016
                : 18 January 2017
                Page count
                Figures: 4, Tables: 2, Equations: 9, References: 55, Pages: 10
                Categories
                Research Article

                cepedea longa,fejervarya limnocharis,morphology,opalinid,ultrastructure

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