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      Increased Expression of Glutathione S- Transferase in Renal Proximal Tubules in the Early Stages of Diabetes: A Study of Type-2 Diabetes in the Akita Mouse Model

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          Abstract

          Background/Aim: The objective of this study was to examine whether the gene expression profile in the kidney is modified by hyperglycemia in the early stage of diabetes. Methods: We analyzed the expression of kidney mRNAs using cDNA array membranes including 588 genes in the kidney of the Akita mouse, a model of type-2 diabetes, after exposure to hyperglycemia for a moderate length of time, but before the manifestation of diabetic glomerulosclerosis. Western blot analysis and immunohistochemical studies were performed to confirm whether the protein for the increasingly expressed mRNA was highly expressed in the kidney of the diabetic mouse. Results: Two of the 10 detected mRNAs, glutathione S-transferase (GST) α and µ, in the kidneys from diabetic mice showed a more than twofold increased expression in comparison to those of control mice. Western blot analysis in kidney tissue extracts confirmed increases in GST α and µ at protein levels in the diabetic mice. Immunohistochemical studies revealed strong staining for those proteins in the proximal tubules of diabetic mice. Conclusion: These data collectively indicate that expression of GSTs is increased in epithelial cells in proximal tubules even at the early stage of diabetes, probably in response to oxidative stress triggered by hyperglycemia or other toxic effects of glucose.

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          A porter with pain in his neck

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            Response of urinary N-acetyl-β-d-glucosaminidase to rapid decreases in blood glucose

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              Author and article information

              Journal
              EXN
              Nephron Exp Nephrol
              10.1159/issn.1660-2129
              Cardiorenal Medicine
              S. Karger AG
              1660-2129
              2001
              2001
              07 November 2001
              : 9
              : 6
              : 380-386
              Affiliations
              Departments of aHygiene, bGeriatric Medicine, and cInternal Medicine III, Akita University School of Medicine, Akita; dDepartment of Health and Environmental Sciences, Kyoto University School of Public Health, Kyoto, Japan
              Article
              52636 Exp Nephrol 2001;9:380–386
              10.1159/000052636
              11701997
              © 2001 S. Karger AG, Basel

              Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

              Page count
              Figures: 5, Tables: 1, References: 21, Pages: 7
              Product
              Self URI (application/pdf): https://www.karger.com/Article/Pdf/52636
              Categories
              Original Paper

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