For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
9
views
36
references
 Top references
cited by
3
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      198
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: not found

      A Novel Experimental Mouse Model of Peritoneal Dissemination of Human Gastric Cancer Cells: Analysis of the Mechanism of Peritoneal Dissemination Using cDNA Macroarrays

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          We established a new cell line, NUGC‐3P4T, with high peritoneal metastatic disseminating potential in nude mice. NUGC‐3P4T cells were derived from the human gastric carcinoma line NUGC‐3, which has low capacity for peritoneal dissemination. NUGC‐3P4T cells developed peritoneal dissemination in 10/10 (100%) mice, whereas the parental NUGC‐3 cells developed dissemination in 1/5 (20.0%) mice. The metastatic foci in the peritoneum showed essentially the same histological appearance as those induced by parental cells. The tumorigenicity, the motile activity and the adhesive activity to the laminin of NUGC‐3P4T cells were stronger than those of NUGC‐3 cells. Production of IL‐8 was significantly higher in NUGC‐3P4T than in NUGC‐3. cDNA macroarrays analysis showed that a variety of cytokines, interleukins, and other immunomodulators and their receptors were up‐ or down‐regulated at the mRNA level in NUGC‐3P4T cells, compared with NUGC‐3 cells. Thus, this unique cell line and in vivo model might be useful to study the biology of peritoneal dissemination of human gastric cancer.

          Related collections

          Most cited references36

          • Record: found
          • Abstract: found
          • Article: not found

          Models of human metastatic colon cancer in nude mice orthotopically constructed by using histologically intact patient specimens.

          A Monosov, Andrew R. Hoffman, X Fu … (1991)
          There is an important need for clinically relevant animal models for human cancers. Toward this goal, histologically intact human colon-cancer specimens derived surgically from patients were implanted orthotopically to the colon or cecum of nude mice. We have observed extensive orthotopic growth in 13 of 20 cases of implanted patient colon tumors. These showed various growth patterns with subsequent regional, lymph-node, and liver metastasis, as well as general abdominal carcinomatosis. Thus, models for human colon cancer have been developed that show (i) local growth, (ii) abdominal metastasis, (iii) general abdominal carcinomatosis with extensive peritoneal seeding, (iv) lymph-node metastasis, (v) liver metastasis, and (vi) colonic obstruction. These models permit the passage of the tumors to form large cohorts. They will facilitate research into the biology of colon cancer metastatic capability and the development of new drugs active against metastatic cancer. These models may also predict the clinical course and the in vivo response to drugs of the cancer of individual patients.
          Article 0 comments Cited 67 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Integrin distribution in malignant melanoma: association of the beta 3 subunit with tumor progression.

            Noa S. Albelda, Bob R. Stewart, S Mette … (1990)
            Since tumor progression is dependent on the ability of malignant cells to interact with the extracellular matrix, molecules on the cell surface which mediate cell-substratum interactions are likely to be important regulators of tumor invasion and metastasis. The purpose of this study was to examine the distribution of one such group of cell adhesion receptors, the integrins, in benign and malignant lesions of human melanocytes. The distribution of integrin adhesion receptors was defined on cells in culture derived from normal and malignant melanocytes and in tissue sections from benign to increasingly malignant melanocytic lesions using a panel of monoclonal antibodies against specific integrin subunits. Cells in culture expressed a large variety of integrins, including all of the previously characterized members of the beta 1 subfamily plus the alpha v/beta 3 vitronectin receptor. The expression of integrins was similar in cells cultured from either benign or malignant lesions. In contrast, consistent differences were noted in integrin expression by cells within tissues containing metastatic and vertical growth phase melanomas when compared to radial growth phase melanoma cells and cells within nevi. Most notably, the expression of the beta 3 subunit was restricted exclusively to cells within vertical growth phase and metastatic melanomas. The presence of this integrin may be important in the development of tumor invasiveness and could be useful as a marker of melanoma cells entering the more aggressive phase of the malignant process.
            Article 0 comments Cited 59 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Milky spots as the implantation site for malignant cells in peritoneal dissemination in mice.

              K Sawai, S Okano, C Sakakura … (1993)
              We examined the site-specific implantation of cancer cells in peritoneal tissues after an i.p. inoculation of 10(5) P388 leukemia cells. Twenty-four h after the inoculation, the number of viable cancer cells infiltrating into specific tissue sites of the peritoneum was estimated by an i.p. transfer method. A descending order of tissue implantation with cancer cells was established as omentum > gonadal fat > mesenterium > posterior abdominal wall > stomach, liver, intestine, anterior abdominal wall, and lung. A significant correlation was established between the logarithm of the number of infiltrating cancer cells and the logarithm of the number of milky spots. Next, the omentum was examined microscopically after i.p. inoculation with P388 leukemia cells labeled with bromodeoxyuridine or B-16 PC melanoma, which were differentiated from the other cells by an immunocytological method using anti-bromodeoxyuridine antibody or by the melanin of the B-16 PC melanoma cells. These cancer cells were found microscopically to be infiltrating only the milky spots, whereas none were seen at the other sites. These results suggest that cancer cells seeded i.p. specifically infiltrate the milky spots in the early stage of peritoneal metastases.
              Article 0 comments Cited 29 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Jpn J Cancer Res
                Journal ID (iso-abbrev): Jpn. J. Cancer Res
                Journal ID (doi): 10.1111/(ISSN)1349-7006a
                Journal ID (publisher-id): CAS
                Title: Japanese Journal of Cancer Research : Gann
                Publisher: Blackwell Publishing Ltd (Oxford, UK )
                ISSN (Print): 0910-5050
                ISSN (Electronic): 1876-4673
                Publication date (Print): July 2001
                Volume: 92
                Issue: 7 ( doiID: 10.1111/cas.2001.92.issue-7 )
                Pages: 748-754
                Affiliations
                [ 1 ]First Department of Surgery, Sapporo Medical University School of Medicine, S‐l W‐16, Chuo‐ku, Sapporo 060‐8543
                [ 2 ]First Department of Pathology, Sapporo Medical University School of Medicine, S‐l W‐16, Chuo‐ku, Sapporo 060‐8543
                [ 3 ]Department of Surgery, Koshin Hospital, 5‐5‐35 Oyachi‐higashi, Atsubetsu‐ku, Sapporo 004‐0041
                Author notes
                [*] [* ] To whom correspondence should be addressed. E‐mail: yasoshima@ 123456mtf.biglobe.ne.jp
                Article
                Publisher ID: CAE748
                DOI: 10.1111/j.1349-7006.2001.tb01157.x
                PMC ID: 5926777
                PubMed ID: 11473725
                SO-VID: 61872eff-6c55-4f7c-9994-2ae15eba91d3
                History
                Page count
                References: 37, Pages: 7
                Categories
                Subject: Article
                Custom metadata
                source-schema-version-number 2.0
                cover-date July 2001
                details-of-publishers-convertor Converter:WILEY_ML3GV2_TO_NLMPMC version:4.6.9 mode:remove_FC converted:04.11.2015

                Keywords: gastric cancer lines,peritoneal dissemination model,nude mice,cdna macroarray
                Data availability:
                Keywords: gastric cancer lines, peritoneal dissemination model, nude mice, cdna macroarray

                Comments

                Comment on this article

                scite_

                Similar content198

                See all similar

                Cited by2

                See all cited by

                Most referenced authors595

                See all reference authors