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      Identification and characterization of major constituents in Juglans mandshurica using ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS)

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          Abstract

          The present study was designed to identify and characterize the major constituents in Juglans mandshurica Maxim. A simple, efficient and sensitive ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-ESI-Q-TOF/MS) method was established and validated under positive and negative ion modes. The separation was performed on a Waters ACQUITY UPLC BEH C 18 column (2.1 mm × 100 mm, 1.7 µm) by gradient elution with a mobile phase (Phase A: 0.1% aqueous formic acid solution, Phase B: 0.1% formic acid acetonitrile solution). A total of 165 compounds were rapidly selected by Targeted and Non-Targeted Peak Finding approaches, and then tentatively identified by comparing with reference substances or inferred through mass spectrometry fragment ion analysis and literature data. These compounds included 68 naphthalenequinones, 20 diarylheptanoids, 29 flavonoids, 20 triterpenes, and 28 phenolic acids. In conclusion, the present study provided an effective approach to identifying components in complex matrices of herbal medicines such as Juglans mandshurica Maxim.

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          Author and article information

          Journal
          CJNM
          Chinese Journal of Natural Medicines
          Elsevier
          1875-5364
          20 July 2018
          : 16
          : 7
          : 525-545
          Affiliations
          1Institute of Chinese Materia Medica, Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China
          2Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin 150040, China
          Author notes
          *Corresponding author: WANG Wei-Ming, Tel: 86-451-55665478, Fax: 86-451-55665478, E-mail: zyyjy@ 123456163.com

          These authors have no conflict of interest to declare.

          Article
          S1875-5364(18)30089-X
          10.1016/S1875-5364(18)30089-X
          Copyright © 2018 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
          Funding
          Funded by: National Natural Science Foundation of China
          Award ID: 81503348
          Funded by: Natural Science Foundation of Heilongjiang Province
          Award ID: H2017044
          Funded by: National Key Subject of Drug Innovation
          Award ID: 2009ZX09102-138
          This work was supported by the National Natural Science Foundation of China (No. 81503348), the Natural Science Foundation of Heilongjiang Province (No. H2017044). and the National Key Subject of Drug Innovation (No. 2009ZX09102-138).

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