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      Molecular mass determination and assay of venom hyaluronidases by sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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      Toxicon

      Elsevier BV

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          Abstract

          We describe a procedure for molecular mass determination of hyaluronidases present in animal venoms from different families. Hyaluronidases can be revealed, following their electrophoretic separation in sodium dodecyl sulfate-polyacrylamide gel containing hyaluronic acid, by incubating the gel in Triton X-100 to remove sodium dodecyl sulfate and restore in situ enzyme activity. This method allows the detection of as little as 0.025 turbidity-reducing units after 2 hr incubation. All the hyaluronidases from the analyzed invertebrate venoms had a mass below 50,000 and showed only one component, while those from vertebrate venoms were more than 60,000 and in many instances contained more than one form.

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          Author and article information

          Journal
          Toxicon
          Toxicon
          Elsevier BV
          00410101
          August 1992
          August 1992
          : 30
          : 8
          : 925-930
          Article
          1523685
          © 1992

          https://www.elsevier.com/tdm/userlicense/1.0/

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