+1 Recommend
1 collections
      • Record: found
      • Abstract: found
      • Article: found

      Characterization of molecular signature of the roots of Paeonia lactiflora during growth

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          The roots of Paeoniae lactiflora Pall. are widely consumed as crude drugs in Asian countries due to their remarkable beneficial health effects. The present research was undertaken to illuminate the dynamic changes in metabolites and enzymes and facilitate selection of the harvesting time when the herb can provide optimum health benefits. P. lactiflora roots were analyzed at 12 stages of growth for monoterpenoid glycosides, phenols, nucleosides, nucleobases, amino acids, and polysaccharides by high-performance liquid chromatography with photodiode array detector, ultra-high pressure liquid chromatography coupled with tandem mass spectrometry, and UV spectrophotometry. The enzyme activities of plant β-glucosidases and esterases were determined by UV methods. The total content of monoterpenoid glycosides and phenols peaked in December. For nucleosides and nucleobases, the highest content appeared in April. The maximum phasic accumulation of the total amino acids took place in March, and the content of total polysaccharides reached a peak value in September. December, April, and March were selected as the appropriate harvesting times for producing natural medicinal or health food products. Plant β-glucosidases and esterases showed the highest activity in December and May, respectively. When the activity of β-glucosidase increased, esterase activity decreased, while the contents of oxypaeoniflora and paeoniflorin increased. When esterase activity increased, the contents of benzoylpaeoniflorin, paeoniflorin, and gallic acid decreased. In conclusion, the results from the present study would be useful in determination of the suitable time for harvesting P. lactiflora roots for medicinal purposes.

          Related collections

          Author and article information

          Chinese Journal of Natural Medicines
          20 October 2017
          : 15
          : 10
          : 785-793
          1Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
          2Institute of Bioengineering, Zhejiang Chinese Medical University, Hangzhou 310053, China
          Author notes
          *Corresponding author: DUAN Jin-Ao, Fax/Tel: 86-25-85811916, E-mail: dja@

          These authors have no conflict of interest to declare.

          Copyright © 2017 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
          Funded by: National Natural Science Foundation of China
          Award ID: 81573555
          Funded by: Natural Science Foundation of Jiangsu Province, China
          Award ID: BK 20160097
          This work was financially supported by National Natural Science Foundation of China (No. 81573555) and Natural Science Foundation of Jiangsu Province, China (No. BK 20160097). We thank Jiangsu Key Laboratory for TCM Formulae Research and Jiangsu Key Laboratory for High Technology Research of TCM Formulae for providing equipment support.


          Comment on this article