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Speckle variance detection of microvasculature using swept-source optical coherence tomography.

Optics letters

methods, Animals, Image Enhancement, Image Interpretation, Computer-Assisted, Mice, Mice, Nude, Microcirculation, Algorithms, cytology, Microscopy, Confocal, Microscopy, Fluorescence, Reproducibility of Results, Sensitivity and Specificity, Tomography, Optical Coherence

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      We report on imaging of microcirculation by calculating the speckle variance of optical coherence tomography (OCT) structural images acquired using a Fourier domain mode-locked swept-wavelength laser. The algorithm calculates interframe speckle variance in two-dimensional and three-dimensional OCT data sets and shows little dependence to the Doppler angle ranging from 75 degrees to 90 degrees . We demonstrate in vivo detection of blood flow in vessels as small as 25 microm in diameter in a dorsal skinfold window chamber model with direct comparison with intravital fluorescence confocal microscopy. This technique can visualize vessel-size-dependent vascular shutdown and transient vascular occlusion during Visudyne photodynamic therapy and may provide opportunities for studying therapeutic effects of antivascular treatments without on exogenous contrast agent.

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