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      Speckle variance detection of microvasculature using swept-source optical coherence tomography.

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          Abstract

          We report on imaging of microcirculation by calculating the speckle variance of optical coherence tomography (OCT) structural images acquired using a Fourier domain mode-locked swept-wavelength laser. The algorithm calculates interframe speckle variance in two-dimensional and three-dimensional OCT data sets and shows little dependence to the Doppler angle ranging from 75 degrees to 90 degrees . We demonstrate in vivo detection of blood flow in vessels as small as 25 microm in diameter in a dorsal skinfold window chamber model with direct comparison with intravital fluorescence confocal microscopy. This technique can visualize vessel-size-dependent vascular shutdown and transient vascular occlusion during Visudyne photodynamic therapy and may provide opportunities for studying therapeutic effects of antivascular treatments without on exogenous contrast agent.

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          Author and article information

          Journal
          Opt Lett
          Optics letters
          Optica Publishing Group
          0146-9592
          0146-9592
          Jul 01 2008
          : 33
          : 13
          Affiliations
          [1 ] Department of Medical Biophysics, University of Toronto, Toronto, M5G 2M9, Canada.
          Article
          165553
          10.1364/ol.33.001530
          18594688
          62162f9b-adaf-4617-bed3-00870ae305ab
          History

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