Author Contributions S.G.F.R. performed the final stages of β
2AR purification; assisted with β
2AR and Gs protein virus production and expression in insect cell cultures; worked
out conditions to form and stabilize the β
2AR-Gs complex following screening, identification and characterization of the BI-167107
agonist and MNG-3 detergent; developed the β
2AR-Gs complex purification strategy with B.K.K. and characterized the stability of
the complex under a variety of conditions; purified and analyzed all preparations
of the β
2AR-Gs complex used for crystallography, DXMS and EM studies, immunization, and nanobody
selection; expressed and purified nanobodies and characterized β
2AR-Gs-Nb binding by size exclusion chromatography; set up crystallization trials in
detergent solution, bicelles and lipidic cubic phase; crystallized the T4L-β
2AR-Gs, T4L-β
2AR-Gs-Nb37 and T4L-β
2AR-Gs-Nb35 complexes; optimized crystallization conditions and grew crystals for data
collection; assisted with data collection and manuscript preparation.
B.T.D. managed Gs heterotrimer subunit virus production and titration; expressed and
purified Gs protein; with R.K.S. he identified the use of apyrase in forming the β
2AR-Gs complex and foscarnet/pyrophosphate during crystallogenesis; reconstituted the
β
2AR-Gs complex and receptor alone in HDL particles which were used for the initial
nanobody selection. He assisted with data collection.
Y.Z. designed, generated and optimized the T4L-β
2AR fusion protein, characterized its expression and functional properties, and prepared
baculovirus for large scale expression.
A.C.K. harvested crystals, collected and processed diffraction data, solved and refined
the structure, and assisted with manuscript preparation.
K.Y.C. developed the cross-linking conditions for the purified β
2AR-Gs complex used for immunization of llamas.
E.P. performed llama immunization, cloned and expressed nanobodies and performed initial
selections.
J.S. supervised nanobody production.
D.C. assisted with Gs heterotrimer expression and purification.
J.M.M. generated the β
2AR-Gs peptide fusion construct, expressed it in insect cell membranes and performed
competition binding experiments.
F.S.T. expressed β
2AR in insect cell cultures and with T.S.K. performed the initial stage of β
2AR purification.
S.T.A.S., J.A.L., and M.C. provided the 7.7 MAG lipid and helpful suggestions for
lipidic mesophase crystallization using this lipid.
P.S.C. and S.H.G. provided MNG-3 detergent for stabilization of the β
2AR-Gs complex.
G.S. provided the essential feedback from the electron microscopy studies that helped
guide the crystallization effort.
W.I.W. oversaw data processing, structure determination and refinement.
R.K.S. supervised Gs protein production, provided valuable ideas and insights into
Gs structure and function, and assisted with data collection and manuscript preparation.
B.K.K. was responsible for overall project strategy and management, harvested crystals
and assisted with collection of diffraction data, and wrote the manuscript.