Objective To detect the expression of interleukin–6 (IL-6) in plasma samples from different populations, and we explore its clinical significance in the diagnosis of tuberculosis.
Methods Using protein chip to screen the diagnostic markers of tuberculosis, then the results showed that the level of IL-6 in the plasma of tuberculosis patients was significantly increased. The expanded samples were measured by enzyme-linked immunosorbent assay (ELISA) in 39 laboratory-confirmed tuberculosis groups , 31 tuberculosis latent infection groups (LTBI), and 31 healthy controls (HC), to evaluate the efficacy of IL- 6 to tuberculosis.
Results Protein microarray assay showed that the expression of IL-6 in TB group, LTBI group and HCTB group was (550.39±479.82) pg/mL, (16.67± 12.95) pg/mL and (126.89±84.75) pg/mL, respectively, which was significantly higher in TB group than in LTBI group and HC group (P<0.01). By ELISA, the expressions in TB group, LTBI group and HCTB group were (8 359.85±7 487.13) pg/mL, (1 102.54±2 079.32) pg/mL and (1 873.60±4 320.03) pg/mL, respectively, which were significantly higher in TB group than in LTBI group and HC group (P<0.01). The results showed that the expression level of IL- 6 in plasma was significantly increased in the TB group, and significantly decreased in the LTBI group and the HCTB group. The area under curve(AUC) of TB and HC was identified as 0.857 7, the sensitivity was 79.49%, and the specificity was 80.65%. The efficacy AUC of the TB and LTBI was 0.883 4, the sensitivity was 92.31%, and the specificity was 70.97%. IL-6 plasma level was higher in the TB patients than healthy subjects and the latent tuberculosis patients (P<0.01).
Conclusion IL-6 plasma level was higher in the TB patients than healthy subjects and the latent tuberculosis patients. IL-6 may be used as a biomarker for distinguishing between active tuberculosis and latent tuberculosis infection for early diagnosis.
摘要： 目的 的检测不同人群血浆标本中白细胞介素-6(IL-6)的表达情况, 探讨其在结核病诊断中的临床意义。 方法以蛋白芯片筛选结核诊断标志物时发现IL-6在结核患者中高表达, 扩大样本采用酶联免疫吸附试验(ELISA)法检 测 39例实验室确诊结核病组(patients with tuberculosis, TB)、31 例结核潜伏感染组 (latent tuberculosis infection, LTBI)及31 例健康对照组(healthy control, HC)3组人群血浆中IL-6水平的差异, 以评价IL-6对结核病的效能。 结果 蛋白芯片检测 发现IL-6在TB组、LTBI组和 HCTB组中表达分别为(550.39±4 79.82)pg/mL、(16.6 7±12.95) pg/mL、(126.89±84.75) pg/mL, TB组明显高于LTBI组及HC组, 差异有统计学意义(P<0.01)。以ELISA, TB组、LTBI组和HCTB组中表达分别为 (8 359.85±7 487.13) pg/mL、(1 102.54±2 079.32) pg/mL、(1 873.60±4 320.03) pg/mL, TB 组明显高于 LTBI组及HC 组, 差异 有统计学意义(P<0.01)。分辨TB和HC的效能曲线下面积(Area Under Curve, AUC)为0.857 7, 灵敏度为79.49%, 特异 度为80.65%;分辨TB和LTBI的效能AUC为0.883 4, 灵敏度为92.31%, 特异度为70.97%。结核患者血浆中IL-6的表达 水平显著高于健康对照和结核潜伏感染人群(P<0.01)。结论结核患者血浆中IL-6的表达显著高于健康对照和潜伏 感染者, IL-6可能作为用于区分活动性结核与潜伏结核感染人群的生物标识, 用于早期诊断。