+1 Recommend
0 collections
      • Record: found
      • Abstract: found
      • Article: not found

      Role of the biofilm master regulator CsgD in cross-regulation between biofilm formation and flagellar synthesis.

      Journal of Bacteriology

      metabolism, Artificial Gene Fusion, Bacterial Proteins, biosynthesis, Binding Sites, Biofilms, growth & development, Chromatin Immunoprecipitation, Consensus Sequence, DNA Footprinting, DNA, Bacterial, genetics, Escherichia coli, physiology, Escherichia coli Proteins, Flagella, Gene Expression Regulation, Bacterial, Genes, Reporter, Oligonucleotide Array Sequence Analysis, methods, Promoter Regions, Genetic, Protein Binding, Regulon, Trans-Activators, beta-Galactosidase

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          CsgD, the master regulator of biofilm formation, activates the synthesis of curli fimbriae and extracellular polysaccharides in Escherichia coli. To obtain insights into its regulatory role, we have identified a total of 20 novel regulation target genes on the E. coli genome by using chromatin immunoprecipitation (ChIP)-on-chip analysis with a high-density DNA microarray. By DNase I footprinting, the consensus CsgD-binding sequence predicted from a total of 18 target sites was found to include AAAAGNG(N(2))AAAWW. After a promoter-lacZ fusion assay, the CsgD targets were classified into two groups: group I genes, such as fliE and yhbT, are repressed by CsgD, while group II genes, including yccT and adrA, are activated by CsgD. The fliE and fliEFGH operons for flagellum formation are directly repressed by CsgD, while CsgD activates the adrA gene, which encodes an enzyme for synthesis of cyclic di-GMP, a bacterial second messenger, which in turn inhibits flagellum production and rotation. Taking these findings together, we propose that the cell motility for planktonic growth is repressed by CsgD, thereby promoting the switch to biofilm formation.

          Related collections

          Author and article information



          Comment on this article