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      Differential Effects of Three Acute Stressors on the Serotonin 5-HT 1A Receptor System in Rat Brain

      ,

      Neuroendocrinology

      S. Karger AG

      Serotonin, Serotonin receptor, Stress, Adrenal steroids, In situ hybridization

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          Abstract

          The effects of different acute stressors on circulating corticosterone levels, 5-HT <sub>1A</sub> receptors and 5-HT<sub>1A</sub> mRNA levels were measured in male Sprague-Dawley rats. Two hours restraint stress, short swim stress (15 min) and long swim stress (30 min) increased circulating corticosterone levels 10-, 13- and 18-fold, respectively, when measured immediately after termination of the stress. Each stressor produced a unique profile of changes in 5-HT<sub>1A</sub> receptors measured in coronal sections 24 h after the termination of stress with the antagonist [<sup>125</sup>I]-4-(2’-methoxyphenyl)-1-[2’-(n-2’’-pyridinyl)-p-iodobenzamido]ethylpiperazine and the agonist [<sup>3</sup>H]-8-hydroxy-2-(di-n-propylamino)tetralin. Restraint stress produced decreases in antagonist binding in the CA3 region and dentate gyms; agonist binding was decreased only in the dentate gyms. Despite the larger elevation in circulating corticosterone level measured after short swim stress, no changes in agonist or antagonist binding were detected after this stressor. In contrast, the long swim stress increased antagonist binding in the CA2 region and in layers IV–VI of the cortex; agonist binding was also increased in all regions of the hippocampus and in layers I–VI of the cortex. Thus, restraint and long swim stress produce opposite effects on 5-HT <sub>1A</sub> receptor expression in different subregions of the hippocampus. Analysis of presynaptic 5-HT<sub>1A</sub> receptors in the raphe nuclei revealed an increase in antagonist binding in the dorsal raphe following long swim stress. No change in the level of 5-HT<sub>1A</sub> mRNA measured in adjacent sections was detected following any of the stressors. The role of corticosteroid receptors in these stress-induced alterations of 5-HT<sub>1A</sub> receptors and the potential significance of these alterations in the context of affective disorders are discussed.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1997
          1997
          09 April 2008
          : 65
          : 4
          : 246-258
          Affiliations
          Department of Pharmacology, University of Pennsylvania, Philadelphia, Pa., USA
          Article
          127182 Neuroendocrinology 1997;65:246–258
          10.1159/000127182
          9142996
          © 1997 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 13
          Categories
          Physiology of Hypothalamic Neurons

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