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      Quantification of Apoptotic Cells Using Isolated Glomeruli

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          Abstract

          In order to detect apoptotic cells in the kidney, the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method is used in tissue sections. But the number of apoptotic cells per glomerulus in several experimental models of nephritides is about 1 per single tissue section even at peak levels. In this study, we have reported that the TUNEL method and immunostaining of cell-specific markers to a whole isolated glomerulus in combination with laser scan microscopy are potentially useful methods for the analysis of cell turnover within glomeruli.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1997
          1997
          23 December 2008
          : 77
          : 4
          : 474-478
          Affiliations
          Departments of aCell Biology and bCellular Physiology, Institute of Nephrology, cDepartment of Internal Medicine II, Niigata University School of Medicine, Niigata, Japan
          Article
          190327 Nephron 1997;77:474–478
          10.1159/000190327
          9434072
          6298ae1e-26a9-4cde-b0bf-5e4c43cc103f
          © 1997 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 20 March 1997
          Page count
          Pages: 5
          Categories
          Short Communication

          Cardiovascular Medicine,Nephrology
          Apoptosis,Isolated glomeruli,TUNEL method,Laser scan microscopy
          Cardiovascular Medicine, Nephrology
          Apoptosis, Isolated glomeruli, TUNEL method, Laser scan microscopy

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