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      Experimental Corneal Calcification. A Study of Acid Mucopolysaccharides

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          Abstract

          Experimental calcification of the cornea was induced in a group of rabbits following treatment with dihydrotachysterol (DHT) and perfusion of the anterior chamber. This method proved to be simple and reproducible. Perfusion of the anterior chamber with permanganate evoked remarkable corneal edema, in which results of all used histochemical reactions (Alcian blue staining at pH 2.5, colloidal Fe<sup>3+</sup>, Alcian blue in solutions containing increasing concentrations of MgCl<sub>2</sub>, Toluidine blue staining at pH 4.5) were distinctly accentuated. The biochemical determination of hexosamines and glucuronic acid showed the actual decrease in the content of acid mucopolysaccharides in the edematous cornea. Calcification was observed in rabbits treated with DHT since the fourth day, in the superficial corneal stromal lamellae, since the sixth day also in the area of basal membrane of corneal epithelium. In the later intervals both areas confluenced.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1970
          1970
          27 November 2009
          : 1
          : 3
          : 175-186
          Affiliations
          Czechoslovak Academy of Sciences, Laboratory of Physiology and Pathology of the Eye, Prague (Director: Prof. MUDr F. Vrabec, DrSc)
          Article
          264515 Ophthal Res 1970;1:175–186
          10.1159/000264515
          © 1970 S. Karger AG, Basel

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          Page count
          Pages: 12
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