+1 Recommend
1 collections
      • Record: found
      • Abstract: found
      • Article: found

      Experimental Corneal Calcification. A Study of Acid Mucopolysaccharides

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          Experimental calcification of the cornea was induced in a group of rabbits following treatment with dihydrotachysterol (DHT) and perfusion of the anterior chamber. This method proved to be simple and reproducible. Perfusion of the anterior chamber with permanganate evoked remarkable corneal edema, in which results of all used histochemical reactions (Alcian blue staining at pH 2.5, colloidal Fe<sup>3+</sup>, Alcian blue in solutions containing increasing concentrations of MgCl<sub>2</sub>, Toluidine blue staining at pH 4.5) were distinctly accentuated. The biochemical determination of hexosamines and glucuronic acid showed the actual decrease in the content of acid mucopolysaccharides in the edematous cornea. Calcification was observed in rabbits treated with DHT since the fourth day, in the superficial corneal stromal lamellae, since the sixth day also in the area of basal membrane of corneal epithelium. In the later intervals both areas confluenced.

          Related collections

          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          27 November 2009
          : 1
          : 3
          : 175-186
          Czechoslovak Academy of Sciences, Laboratory of Physiology and Pathology of the Eye, Prague (Director: Prof. MUDr F. Vrabec, DrSc)
          264515 Ophthal Res 1970;1:175–186
          © 1970 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 12


          Comment on this article