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Polyadenylated RNA isolated from the archaebacterium Halobacterium halobium.

Journal of Bacteriology

RNA, Messenger, isolation & purification, RNA, Poly A, genetics, Halobacterium, Euryarchaeota, Escherichia coli, Electrophoresis, Agar Gel

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      Abstract

      Polyadenylated [poly(A)+] RNA has been isolated from the halophilic archaebacterium Halobacterium halobium by binding, at 4 degrees C, to oligo(dT)-cellulose. H. halobium contains approximately 12 times more poly(A) per unit of RNA than does the methanogenic archaebacterium Methanococcus vannielii. The 3' poly(A) tracts in poly(A)+ RNA molecules are approximately twice as long (average length of 20 nucleotides) in H. halobium as in M. vannielii. In both archaebacterial species, poly(A)+ RNAs are unstable.

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      214661
      2422156

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