Abstract. This article aims to present the development and test the psychometric properties of the Perceived Occupational Stress (POS) scale, a new brief instrument aimed at rating a worker’s perception of feeling stressed at work. Six studies are conducted on an overall sample of 1,805 Italian workers, to examine both the construct and concurrent validity of the POS scale. The results demonstrate the high internal consistency (α = .82) and test-retest reliability ( r = .86) of the POS scale, as well as its structural validity and concurrent validity with the Maslach Burnout Inventory ( r = .68 with Emotional Exhaustion) and the Effort-Reward Imbalance Questionnaire ( r = .62 with Imbalance and r = .51 with Overcommitment). Moreover, the POS scale is determined to uniquely contribute toward predicting stress-related health complaints, over and above indicators of workplace stressors, as measured by the Health and Safety Executive Management Standards Indicator Tool ( R 2 change = .06). Overall, the present findings indicate that the POS scale is a valid and reliable instrument for self-reporting occupational stress levels, and it could be used together with existing risk assessment measures of stress, to obtain a comprehensive evaluation of work-related stress.