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      A generic protein purification method for protein complex characterization and proteome exploration.

      Nature biotechnology

      chemistry, Affinity Labels, Proteome, isolation & purification, Proteins, Molecular Sequence Data, Methods, Electrophoresis, Polyacrylamide Gel, Amino Acid Sequence

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          Abstract

          We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.

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          Journal
          10.1038/13732
          10504710

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