Mark-release-recapture experiments were conducted to determine the duration of the gonotrophic cycle of Anopheles albitarsis s.l. in Ribeira Valley, State of Sao Paulo, Brazil. Separate experiments with females collected from the field were followed under laboratory conditions. A mean of 2.4 d was characteristic for the gonotrophic cycle for wild-caught females, whereas 4.4 d was characteristic for females in the laboratory conditions.