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      Effects of Centrally Administered Pituitary Adenylate Cyclase-Activating Polypeptide on c- fos Gene Expression and Heteronuclear RNA for Vasopressin in Rat Paraventricular and Supraoptic Nuclei

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          The effects of intracerebroventricular (i.c.v.) administration of pituitary adenylate cyclase-activating polypeptide (PACAP) on the expression of c-fos gene as well as heteronuclear (hn) RNA for arginine vasopressin (AVP) in paraventricular (PVN) and supraoptic nuclei (SON) of rats were investigated by immunohistochemistry for c-fos protein (Fos) and in situ hybridization histochemistry for c-fos mRNA and AVP hnRNA. The i.c.v. administration of PACAP (200 pmol/rat) caused a marked induction of Fos-like immunoreactivity (LI) in PVN and SON. The nuclear Fos-LI existed in AVP-LI containing cells in the PVN and SON. The expression of the c-fos gene in the PVN and SON was increased in a dose-related manner 30 min after i.c.v. administration of PACAP. PACAP-induced expression of the c-fos gene was significantly reduced by pretreatment with a PACAP receptor antagonist, PACAP-(6-38)-NH<sub>2</sub>. In addition, Fos-LI and the expression of the c-fos gene were also observed in the periventricular region of the third ventricle after i.c.v. administration of PACAP. The induction of c-fos gene expression in the PVN and SON reached a maximum 30 min after PACAP administration. The expression of c-fos gene in the PVN and SON induced by i.c.v. administration of vasoactive intestinal peptide (200 pmol/rat) was weaker than that induced by PACAP. The hnRNA for AVP in the PVN and SON was significantly increased 30 min after i.c.v. administration of PACAP (200 pmol/rat). Our results suggest that PACAP activates PVN and SON neurons via PACAP receptors and, in parallel, transcription of the AVP gene in the PVN and SON.

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          The immunocytochemical localization of somatostatin-containing neurons in the rat central nervous system

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            Stress-induced activation of CRF and c-fos mRNAs in the paraventricular nucleus are not affected by serotonin depletion

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              Pituitary adenylate cyclase-activating polypeptide: a novel, long-lasting, endothelium-independent vasorelaxant


                Author and article information

                S. Karger AG
                March 1999
                19 March 1999
                : 69
                : 3
                : 167-180
                Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan
                54416 Neuroendocrinology 1999;69:167–180
                © 1999 S. Karger AG, Basel

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                Page count
                Figures: 14, References: 44, Pages: 14
                Regulation of Hypothalamic Neurons


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