A Graphene‐Based Platform for the Assay of Duplex‐DNA Unwinding by Helicase †

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Translated abstract

Mit bloßen Auge zu erkennen: Mithilfe von Graphenoxid (GO) kann die Helicase‐abhängige Entwindung doppelsträngiger DNA (dsDNA) quantitativ und in Echtzeit verfolgt werden. GO bindet selektiv an entwundene fluoreszenzmarkierte einzelsträngige DNA und löscht die Fluoreszenz (siehe Bild). Die Helicaseaktivität wird über die Fluoreszenzänderung registriert.

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Most cited references 1

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Strategies for antiviral drug discovery.

Sarah Rowland-Jones (1998)

The need for antiviral drugs is growing rapidly as more viral diseases are recognized. The methods used to discover antiviral drugs have evolved considerably over the past 40 years and the overall process of discovery can be broken down into subprocesses which include lead generation, lead optimization and lead development. Various methods are now employed to ensure these processes are carried out efficiently. For lead generation, screening methodologies have developed to the extent where hundreds of thousands of compounds can be screened against a particular target. An alternative approach is to use the structures of enzyme substrates as a starting point for drug discovery. Much use is now made of X-ray crystallographic data of target-inhibitor complexes for the optimization of lead structures, and methods for preparing libraries of compounds to assist both generation and optimization of leads are well-developed. The methods used to predict and improve the pharmacokinetic properties of compounds are also changing rapidly. Finally, novel approaches to antiviral therapy using oligonucleotide-based compounds or by modulating the host immune response are also being explored. This review discusses these approaches, provides examples of where their application has been successful and sets them against a historical background.

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Author and article information

Contributors

Dal‐Hee Min: dalheemin@kaist.ac.kr

Journal

Journal ID (nlm-ta): Angew Chem Weinheim Bergstr Ger

Journal ID (iso-abbrev): Angew Chem Weinheim Bergstr Ger

Journal ID (doi): 10.1002/(ISSN)1521-3757

Journal ID (publisher-id): ANGE

Title: Angewandte Chemie (Weinheim an Der Bergstrasse, Germany)

Publisher: WILEY‐VCH Verlag (Weinheim )

ISSN (Print): 0044-8249

ISSN (Electronic): 1521-3757

Publication date (Electronic): 07 July 2010

Publication date (Print): 02 August 2010

Volume: 122

Issue: 33 ( doiID: 10.1002/ange.v122:33 )

Pages: 5839-5843

Affiliations

[ ¹ ]Department of Chemistry, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), 373‐1 Guseong‐dong, Yuseong‐gu, Daejeon 305‐701 (Korea), Fax: (+82)‐42‐350‐2810

[ ² ]Department of Bioscience and Biotechnology, Konkuk University, Seoul 143‐701 (Korea)

Article

Publisher ID: ANGE201001332

DOI: 10.1002/ange.201001332

PMC ID: 7159641

SO-VID: 63aa2f8e-5b7a-4c5a-9d7d-e60466328ccf

License:

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

History

Date received : 05 March 2010

Date revision received : 19 April 2010

Page count

Figures: 7, Tables: 0, References: 51, Pages: 5

Funding

Funded by: National Research Foundation of Korea (NRF)

Funded by: Ministry of Education, Science, and Technology, MEST

Award ID: 313‐2008‐2‐C00538

Award ID: 2008‐0062074

Award ID: 2009‐0071058

Funded by: Nano R&D program

Award ID: 2008‐2004457

Funded by: WCU Project

Award ID: R33‐10128

Funded by: National Honor Scientist Program

Custom metadata

source-schema-version-number 2.0

cover-date August 2, 2010

details-of-publishers-convertor Converter:WILEY_ML3GV2_TO_JATSPMC version:5.8.0 mode:remove_FC converted:15.04.2020

Keywords: biosensoren,dna,fluoreszenzspektroskopie,graphen,viren

Data availability:

Keywords: biosensoren, dna, fluoreszenzspektroskopie, graphen, viren

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A Graphene‐Based Platform for the Assay of Duplex‐DNA Unwinding by Helicase†

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