13
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Chemerin inhibition of myogenesis and induction of adipogenesis in C2C12 myoblasts.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Chemerin is an adipocyte-secreted adipokine that regulates the differentiation and metabolism of adipose through auto-/paracrine signaling. Its function in the differentiation of multipotent myoblast cells has thus far received little attention. In this study, C2C12 myoblast cells were cultured in the medium with Chemerin, and the differentiation potential of C2C12 myoblasts was analyzed. The results showed that Chemerin increased ROS levels and TG content of C2C12 cells. At the same time, the mRNA expressions and protein concentrations of the adipogenic factors PPARγ, C/EBPα and UCP1 were up-regulated, while the muscle specific transcription factors MyoD, Myogenin and MyHC were decreased in cultured C2C12 cells. In conclusion, the adipokine Chemerin promoted the adipogenic differentiation potential and altered the fate of myoblast cells from myogenesis to adipogenesis, which contributed in part to the up-regulated adipocyte genes. Our study reveals the importance of functional Chemerin signaling in adipogenesis and in directing the differentiation of multipotent myoblast cells.

          Related collections

          Author and article information

          Journal
          Mol. Cell. Endocrinol.
          Molecular and cellular endocrinology
          Elsevier BV
          1872-8057
          0303-7207
          Oct 15 2015
          : 414
          Affiliations
          [1 ] College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: lihuixia@njau.edu.cn.
          [2 ] College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
          [3 ] Department of Animal Sciences, Chungbuk National University, Naesudong-ro, Seowon-gu, Cheongju-si 362-763, Chungcheongbuk-do, Republic of Korea.
          [4 ] National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China; Synergetic Innovation Center of Food Safety and Nutrition, Nanjing Agricultural University, Nanjing 210095, China.
          [5 ] National Center of Meat Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China; Synergetic Innovation Center of Food Safety and Nutrition, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: ghzhou@njau.edu.cn.
          Article
          S0303-7207(15)30015-0
          10.1016/j.mce.2015.07.006
          26164089
          63ba541c-a8ef-42f0-81e3-9a0f91b0574b
          History

          Myogenesis,Adipogenesis,Brown adipose,C2C12 myoblast cells,Chemerin

          Comments

          Comment on this article