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      A reusable capacitive immunosensor for detection of Salmonella spp. based on grafted ethylene diamine and self-assembled gold nanoparticle monolayers

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      Analytica Chimica Acta
      Elsevier BV

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          Abstract

          Fabrication of a novel capacitive immunosensor based on grafted ethylene diamine and self-assembled gold nanoparticle monolayer on glassy carbon electrode for the detection of Salmonella spp. is described for the first time. In the present study, the Salmonella spp. monoclonal antibodies (denoted as McAbs) was immobilized on gold nanoparticles. Interaction of McAbs and Salmonella spp. was detected directly using the electrochemical impedance spectroscopy (EIS) technique. The experimental results showed that the concentration of antigen was measured through the relative change in capacitance in the corresponding specific binding of Salmonella spp. and McAbs. Under the optimized conditions, the relative changes in capacitance were proportional to the logarithmic values of Salmonella spp. concentrations in the range of 1.0 x 10(2) to 1.0 x 10(5) CFU mL(-1) (r = 0.991) with the detection limit of 1.0 x 10(2) CFU mL(-1). The stability of proposed immunosensor could be estimated by determining the relative change in capacitance, which remained almost the same in two months and decreased gradually to 85.3% of initial value after four months' storage. The used immunosensor could be regenerated repeatedly by immersing in glycine-HCl buffer solution (pH 2.8). Finally, the proposed immunosensor was successfully used for the detection of Salmonella spp. in lab-processed commercial pork samples.

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          Author and article information

          Journal
          Analytica Chimica Acta
          Analytica Chimica Acta
          Elsevier BV
          00032670
          August 2009
          August 2009
          : 647
          : 2
          : 159-166
          Article
          10.1016/j.aca.2009.06.008
          19591700
          63f69efb-ef78-4309-9bdb-1eff6a3be594
          © 2009

          https://www.elsevier.com/tdm/userlicense/1.0/

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