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      Molecular and morphological identification of Biomphalaria species from the state of São Paulo, Brazil

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          Abstract

          Abstract

          DNA barcoding and morphological characters were used to identify adult snails belonging to the genus Biomphalaria from 17 municipalities in the state of São Paulo, Brazil. The DNA barcode analysis also included twenty-nine sequences retrieved from GenBank. The final data set of 104 sequences of the mitochondrial cytochrome oxidase I (COI) gene was analyzed for K2P intraspecific and interspecific divergences, through tree-reconstruction methods (Neighbor-Joining, Maximum Likelihood and Bayesian inference), and by applying different models (ABGD, bPTP, GMYC) to partition the sequences according to the pattern of genetic variation. Twenty-seven morphological parameters of internal organs were used to identify specimens. The molecular taxonomy of Biomphalaria agreed with the morphological identification of specimens from the same collection locality. DNA barcoding may therefore be a useful supporting tool for identifying Biomphalaria snails in areas at risk for schistosomiasis.

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          MRBAYES: Bayesian inference of phylogenetic trees.

          The program MRBAYES performs Bayesian inference of phylogeny using a variant of Markov chain Monte Carlo. MRBAYES, including the source code, documentation, sample data files, and an executable, is available at http://brahms.biology.rochester.edu/software.html.
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            The promise of DNA barcoding for taxonomy.

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              Estado atual da sistemática dos planorbídeos Brasileiros

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                Author and article information

                Journal
                Zookeys
                Zookeys
                ZooKeys
                ZooKeys
                Pensoft Publishers
                1313-2989
                1313-2970
                2017
                12 April 2017
                : 668
                : 11-32
                Affiliations
                [1 ] Department of Epidemiology, Faculty of Public Health, University of São Paulo, Pinheiros, SP, Brazil
                [2 ] Biochemistry and Molecular Biology Laboratory, Superintendency for the Control of Endemic Diseases, Rua Paula Sousa, 166, Luz, São Paulo, SP, Brazil
                [3 ] Malacology Laboratory, Regional Service SR-02, Superintendency for the Control of Endemic Diseases, São Vicente, Rua João Ramalho, 587, Centro, SP, Brazil
                [4 ] Malacology Laboratory, Special Programs Division SR-01, Superintendency for the Control of Endemic Diseases, Rua Cardeal Arcoverde, 2878 - Pinheiros, SP, Brazil
                Author notes
                Corresponding author: Roseli Tuan ( rtuan@ 123456sucen.sp.gov.br ; roselituan@ 123456gmail.com )

                Academic editor: R. Hershler

                Article
                10.3897/zookeys.668.10562
                5534528
                63fa4606-3ed9-402c-988f-6a5748804f49
                Raquel Gardini Sanches Palasio, Marisa Cristina de Almeida Guimarães, Fernanda Pires Ohlweiler, Roseli Tuan

                This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 10 October 2016
                : 21 March 2017
                Funding
                Funded by: Fundação de Amparo à Pesquisa do Estado de São Paulo 501100001807 http://doi.org/10.13039/501100001807
                Categories
                Research Article

                Animal science & Zoology
                biomphalaria,coi,dna barcoding,morphological taxonomy,schistosomiasis,species identification

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