Ferns are underestimated as medicinal plants, and their use in traditional medicine is limited despite their diversity. Most of the research on their healing properties and phytochemical composition related to their biological activity has been focused on the secondary metabolites synthesized by ferns.
In this study, we aimed to make an in-depth chemical characterization of three ferns widely spread in Bulgaria – Asplenium ceterach L., Asplenium scolopendrium L., and Asplenium trichomanes L.
Micro elemental analysis was carried out using ICP-MS. Standard laboratory methods were used to determine the content of proteins, fats, and fatty acids. Chromatographic methods were applied to quantify some secondary metabolites. Two antioxidant methods and two antimicrobial tests were used to evaluate the biological properties of the ferns tested.
Micro elemental analysis showed that these ferns could be a useful source of zinc and iron. Protein content slightly varied among the species (13.6% – 18.4% of the dried plant weight). Thevariation in oil content was negligible (3.1% – 4.0%, dry weight base); oil composition was dominated by saturated fatty acids. Some principal classes of secondary metabolites, individual phenolic acids, and flavonoids were quantified. The extract obtained from Asplenium ceterach L. demonstrated the highest antioxidant capacity. Fern extracts showed moderate antimicrobial activity against six of the 14 microorganisms tested.