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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

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Analytical biochemistry

Elsevier BV

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      Abstract

      A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.

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      Journal
      Anal. Biochem.
      Analytical biochemistry
      Elsevier BV
      0003-2697
      0003-2697
      Apr 1987
      : 162
      : 1
      2440339
      10.1006/abio.1987.9999
      0003-2697(87)90021-2

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