Blog
About

  • Record: found
  • Abstract: found
  • Article: not found

Basic local alignment search tool.

Read this article at

ScienceOpenPublisherPubMed
Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Abstract

      A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score. Recent mathematical results on the stochastic properties of MSP scores allow an analysis of the performance of this method as well as the statistical significance of alignments it generates. The basic algorithm is simple and robust; it can be implemented in a number of ways and applied in a variety of contexts including straightforward DNA and protein sequence database searches, motif searches, gene identification searches, and in the analysis of multiple regions of similarity in long DNA sequences. In addition to its flexibility and tractability to mathematical analysis, BLAST is an order of magnitude faster than existing sequence comparison tools of comparable sensitivity.

      Related collections

      Author and article information

      Affiliations
      [1 ] National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894.
      Journal
      J. Mol. Biol.
      Journal of molecular biology
      0022-2836
      0022-2836
      Oct 5 1990
      : 215
      : 3
      2231712
      10.1016/S0022-2836(05)80360-2
      S0022-2836(05)80360-2

      Comments

      Comment on this article