Applying Both Chemical Liquefaction and Enzymatic Catalysis Can Increase Production of Agaro-Oligosaccharides from Agarose

Abstract

Red algae represents an important marine bioresource. One high-value utilization of red algae is the production of agarooligosaccharides which have many positive biological effects. However, the lack of an efficient production route seriously limits the application of agaro-oligosaccharides. In this study, we established a green route that combines chemical liquefaction and enzymatic catalysis for the efficient production of agaro-oligosaccharides, and we used the production of neoagarotetraose (NA4) as an example. Agarose (150 g L ⁻¹) liquefaction by citric acid was controlled with respect to two targets: a 100% liquefaction rate and a high average degree of polymerization (> 4) of the liquesced agaro-oligosaccharides, which were then catalyzed by β-agarase into an oligosaccharides mixture with a high concentration of NA4 (30.8 g L ⁻¹) in a 1-L reaction volume. After purification, we obtained 25.5 g of NA4 with a purity of 92%. This work establishes an easy route for the efficient production of pure agaro-oligosaccharides from agarose.

Journal

Journal ID (publisher-id): JOUC

Title: Journal of Ocean University of China

Publisher: Science Press and Springer (China )

ISSN (Electronic): 1672-5182

Publication date (Electronic): 15 November 2020

Publication date (Print): 01 December 2020

Volume: 19

Issue: 6

Pages: 1371-1377

Affiliations

¹College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

²Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China

³Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510300, China

Author notes

*Corresponding authors: LIU Zhen, E-mail: liuzhenyq@ 123456ouc.edu.cn ; MAO Xiangzhao, E-mail: xzhmao@ 123456ouc.edu.cn

Article

Publisher ID: s11802-020-4439-8

DOI: 10.1007/s11802-020-4439-8

Copyright statement: Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

License:

The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

Product

Self URI (journal-page): https://www.springer.com/journal/11802