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      Influence of Cellular Energy Metabolism on Contractions of Porcine Carotid Artery Smooth Muscle

      Journal of Vascular Research

      S. Karger AG

      Latch, Smooth muscle, Metabolism, Phosphate, ADP, pH, Mg2+ , Force

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          Abstract

          A number of cellular metabolites, including inorganic phosphate and ADP, have been proposed to regulate the contractions of smooth muscle. Hypothesizing that one of these would have a greater influence than the others, parallel experiments using tissue mechanics and <sup>31</sup>P-NMR allowed comparison of several metabolic components with the generation of force in porcine carotid artery smooth muscle during long-term contractions. P<sub>i</sub>, ADP, ATP, PCr, free energy, pH, and free Mg<sup>2+</sup> were determined from phosphate spectra during a control-hypoxia-postcontrol sequence generated during K<sup>+</sup> stimulation by replacement of oxygen with nitrogen using either pyruvate or glucose as substrate. Both pH and free Mg<sup>2+</sup> were significantly lower in control pyruvate-supplied tissues than in glucose-supplied tissues. Mechanical experiments following the same protocol produced variations in force. The pyruvate series produced the greater range of mechanical and metabolic changes. Linear and logarithmic regression analysis found the order of correlation with force to be highest for P<sub>i</sub>, followed by pH, free energy, PCr, ATP, ADP, and free Mg<sup>2+</sup>. The results are consistent with models for the regulation of myosin ATPase by free phosphate inhibition. The results are inconsistent with models of ADP as a regulator of smooth muscle force. Perturbations which alter intracellular phosphate, such as creatine loading, may produce side effects on the contractions of vascular smooth muscle.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          2000
          December 2000
          10 January 2001
          : 37
          : 6
          : 532-539
          Affiliations
          Department of Physiology, Michigan State University, East Lansing, Mich., USA
          Article
          54086 J Vasc Res 2000;37:532–539
          10.1159/000054086
          11146407
          © 2000 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Figures: 5, Tables: 2, References: 20, Pages: 8
          Categories
          Research Paper

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