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      Analytical Identity of an Inhibitor of Sodium Transport Isolated from Human Serum and Urine

      a , b , c , a

      Nephron

      S. Karger AG

      Inhibition, Sodium transport, Identity, Serum, Urine

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          Abstract

          Inhibitors of sodium transport purified from serum and urine of healthy and uremic subjects show the same elution pattern on columns of a weak anion exchanger (DEAE Sephadex A-25), reverse-phase chromatography (Separon SI C 18) and gel permeation chromatography (Separon Hema 300 Glc). Moreover, their thin-layer chromatographic mobility (DC-Alufolien Cellulose) in nine solvent systems is the same. These identical physicochemical properties document satisfactorily their identity, which permits the use of the urinary inhibitor of sodium transport for structure studies.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1985
          1985
          04 December 2008
          : 39
          : 1
          : 18-20
          Affiliations
          aMedical Bionics Research Institute, bResearch Institute of Experimental Medicine and cInstitute of Experimental Endocrinology, Center of Physiological Sciences, Slovak Academy of Sciences, Bratislava, Czechoslovakia
          Article
          183330 Nephron 1985;39:18–20
          10.1159/000183330
          3969186
          © 1985 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 3
          Categories
          Original Paper

          Cardiovascular Medicine, Nephrology

          Serum, Identity, Sodium transport, Inhibition, Urine

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