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      lncRNA H19/miR-675 axis represses prostate cancer metastasis by targeting TGFBI.

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          Abstract

          Prostate cancer is a leading cause of cancer-related mortality in men worldwide and there is a lack of effective treatment options for advanced (metastatic) prostate cancer. Currently, limited knowledge is available concerning the role of long non-coding RNAs in prostate cancer metastasis. In this study, we found that long non-coding RNA H19 (H19) and H19-derived microRNA-675 (miR-675) were significantly downregulated in the metastatic prostate cancer cell line M12 compared with the non-metastatic prostate epithelial cell line P69. Upregulation of H19 in P69 and PC3 cells significantly increased the level of miR-675 and repressed cell migration; however, ectopic expression of H19 in M12 cells could not increase the level of miR-675 and therefore had no effect on cell migration. Furthermore, we found that the expression level of either H19 or miR-675 in P69 cells was negatively associated with the expression of transforming growth factor β induced protein (TGFBI), an extracellular matrix protein involved in cancer metastasis. Dual luciferase reporter assays showed that miR-675 directly bound with 3'UTR of TGFBI mRNA to repress its translation. Taken together, we show for the first time that the H19-miR-675 axis acts as a suppressor of prostate cancer metastasis, which may have possible diagnostic and therapeutic potential for advanced prostate cancer.

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          Author and article information

          Journal
          FEBS J.
          The FEBS journal
          1742-4658
          1742-464X
          Aug 2014
          : 281
          : 16
          Affiliations
          [1 ] Department of Oncology, No. 3 People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, China; Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory of Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, China.
          Article
          10.1111/febs.12902
          24988946
          65813ed9-5a1a-4515-9d0c-d23350a9d909
          © 2014 FEBS.
          History

          TGFBI,lncRNA H19,miR-675,prostate cancer,tumor metastasis
          TGFBI, lncRNA H19, miR-675, prostate cancer, tumor metastasis

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