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      Allelic exchange in Actinomyces oris with mCherry fluorescence counterselection.

      1 ,

      Applied and environmental microbiology

      American Society for Microbiology

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          Abstract

          Described here is a method for facile generation of markerless gene deletion mutants of Actinomyces oris. Homologous integration of a nonreplicative vector carrying a gene exchange cassette into the bacterial chromosome was selected for by using mCherry fluorescence and resistance to kanamycin. Completion of allelic replacement was counterselected for by using loss of fluorescence.

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          Author and article information

          Journal
          Appl. Environ. Microbiol.
          Applied and environmental microbiology
          American Society for Microbiology
          1098-5336
          0099-2240
          Sep 2010
          : 76
          : 17
          Affiliations
          [1 ] Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, 6431 Fannin Street, Houston, TX 77030, USA.
          Article
          AEM.00811-10
          10.1128/AEM.00811-10
          2935053
          20601506

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