Roberta Lentini 1 , Silvia Perez Santero 1 , 2 , Fabio Chizzolini 1 , Dario Cecchi 1 , Jason Fontana 1 , Marta Marchioretto 3 , Cristina Del Bianco 1 , Jessica L. Terrell 4 , 5 , Amy C. Spencer 1 , Laura Martini 1 , Michele Forlin 1 , Michael Assfalg 2 , Mauro Dalla Serra 3 , William E. Bentley 4 , 5 , Sheref S. Mansy a , 1
30 May 2014
Previous efforts to control cellular behaviour have largely relied upon various forms of genetic engineering. Once the genetic content of a living cell is modified, the behaviour of that cell typically changes as well. However, other methods of cellular control are possible. All cells sense and respond to their environment. Therefore, artificial, non-living cellular mimics could be engineered to activate or repress already existing natural sensory pathways of living cells through chemical communication. Here we describe the construction of such a system. The artificial cells expand the senses of Escherichia coli by translating a chemical message that E. coli cannot sense on its own to a molecule that activates a natural cellular response. This methodology could open new opportunities in engineering cellular behaviour without exploiting genetically modified organisms.
The control of cellular behaviour largely relies on genetic engineering, but artificial cells could be designed to control cell processes through chemical communication. Here, the authors develop an artificial cell that is able to translate a chemical message into a signal that can be sensed by E. coli and activate a cellular response.