This article focuses on the problems connected with fluctuation of nursing staff. Today, the fluctuation of health care staff is a widespread phenomenon. This was further accentuated after the Czech Republic joined the EU as nursing staff started to make greater use of the free movement of people in the labor market. They departed to other countries as well as to more attractive workplaces which were less demanding on their personal, family and work life. It is for this reason that attention is paid to this problem. The main objective was to find out which selected factors influence the fluctuation of nursing staff and which serious factors influence the fluctuation. The chosen survey respondents were nurses working in intensive care units and general wards.