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      Uveal Mast Cells Are Not Required for Rodent Uveitis


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          Uveal mast cells have previously been considered to be vital mediators of experimental uveitis. We extended the study of these cells to experimental melanin-induced uveitis (EMIU), a recently described clinically relevant model, and re-examined their role in endotoxin-induced uveitis (EIU) using genetically mast cell-depleted mice on a single background. EMIU was induced in Fischer 344 rats by immunisation with bovine ocular melanin (250 µg). Animals were killed immediately, and on days 1 and 3 of clinical disease. Numbers of uveal mast cells and the percentage of degranulated cells were counted in whole-mount preparations. There was no significant change in either measure across the selected time points. To induce EIU, normal and mast cell-depleted DBA/2 mice were injected with Escherichia coli lipopolysaccharide (400 µg). Cells infiltrating the eye 24 h after injection were quantified in 5 µm ocular cross-sections. Disease was not significantly reduced in the mast cell-depleted mutants. We conclude that uveal mast cells are not required for the development of EMIU or, in contrast to earlier work, EIU.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          December 1998
          05 October 1998
          : 30
          : 6
          : 388-393
          Departments of a Ophthalmology and b Microbiology and Infectious Diseases, Flinders University of South Australia, Bedford Park, Australia
          55500 Ophthalmic Res 1998;30:388–393
          © 1998 S. Karger AG, Basel

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          Page count
          Figures: 2, Tables: 1, References: 15, Pages: 6
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Mouse,Uveal mast cell,Experimental melanin-induced uveitis,Endotoxin-induced uveitis,Rat


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