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      Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs.

      Genes & development

      metabolism, ran GTP-Binding Protein, genetics, RNA, Messenger, RNA Processing, Post-Transcriptional, RNA Precursors, RNA Interference, chemistry, RNA, Promoter Regions, Genetic, Plasmids, MicroRNAs, Luciferases, Humans, Electrophoretic Mobility Shift Assay, Cell Nucleus, Cell Line, Active Transport, Cell Nucleus, Karyopherins

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          Abstract

          MicroRNAs (miRNAs) are initially expressed as long transcripts that are processed in the nucleus to yield approximately 65-nucleotide (nt) RNA hairpin intermediates, termed pre-miRNAs, that are exported to the cytoplasm for additional processing to yield mature, approximately 22-nt miRNAs. Here, we demonstrate that human pre-miRNA nuclear export, and miRNA function, are dependent on Exportin-5. Exportin-5 can bind pre-miRNAs specifically in vitro, but only in the presence of the Ran-GTP cofactor. Short hairpin RNAs, artificial pre-miRNA analogs used to express small interfering RNAs, also depend on Exportin-5 for nuclear export. Together, these findings define an additional cellular cofactor required for miRNA biogenesis and function.

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          Author and article information

          Journal
          14681208
          305252
          10.1101/gad.1158803

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