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Site-selective chemistry and the attachment of peptides to the surface of a microelectrode array.

Journal of the American Chemical Society

chemistry, Sulfhydryl Compounds, instrumentation, Protein Array Analysis, metabolism, Peptides, Oligopeptides, Microelectrodes, Maleimides, Equipment Design, Copper, Acrylates

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      Abstract

      Peptides have been site-selectively placed on microelectrode arrays with the use of both thiol-based conjugate additions and Cu(I)-coupling reactions between thiols and aryl halides. The conjugate addition reactions used both acrylate and maleimide Michael acceptors. Of the two methods, the Cu(I)-coupling reactions proved far superior because of their irreversibility. Surfaces constructed with the conjugate addition chemistry were not stable at neutral pHs, especially the surface using the maleimide acceptor. Once a peptide was placed onto the array, it could be monitored in "real-time" for its interactions with a biological receptor.

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      Journal
      10.1021/ja308121d
      3950271
      22992158

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